| 英文名 |
23S rRNA (adenine2503-C8)-methyltransferase |
| Example Structure |
 |
| 别名 |
Cfr, Cfr methyltransferase, Cfr rRNA methyltransferase, Cfr-like protein, ClbA, ClbB, ClbC, radical AdoMet rRNA methyltransferase, radical-S-adenosyl-L-methionine enzyme, radical-SAM enzyme, radical-SAM rRNA methyltransferase |
| EC Number |
2.1.1.224 |
| CAS编号 |
|
| Comments |
This enzyme is a member of the ‘AdoMet radical’ (radical SAM) family. S-adenosyl-L-methionine acts as both a radical generator and as the source of the appended methyl group. It contains an [4Fe-4S] cluster [1]. Cfr is an plasmid-acquired methyltransferase that protects cells from the action of antibiotics [1]. The enzyme methylates adenosine at position 2503 of 23S rRNA by a radical mechanism, transferring a CH2 group from S-adenosyl-L-methionine while retaining the hydrogen at the C-8 position of the adenine. Cfr first transfers an CH2 group to a conserved cysteine (Cys338 in Staphylococcus aureus) [7], the generated radical from a second S-adenosyl-L-methionine then attacks the methyl group, exctracting a hydrogen. The formed radical forms a covalent intermediate with the adenine group of the tRNA [8]. The enzyme will also methylate 2-methyladenine produced by the action of EC 2.1.1.192 [23S rRNA (adenine2503-C2)-methyltransferase]. |
| Cofactor |
iron-sulfur |
| History |
|
| Reactions |
2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe- 2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5′-deoxyadenosine + 8-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe- 2S] ferredoxin. |
