| 英文名 |
adenosine-5′-diphospho-5′-[DNA] diphosphatase |
| Example Structure |
![Example Structure of adenosine-5'-diphospho-5'-[DNA] diphosphatase EC#: 3.1.11.7](/wp-content/uploads/enzyme-structure/3.1.11.7.jpg) |
| 别名 |
5′-App5′-DNA adenylate hydrolase, aprataxin, aprataxin-like protein, APTX, DNA-adenylate hydrolase, HNT3, RNA-DNA deadenylase |
| EC Number |
3.1.11.7 |
| CAS编号 |
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| Comments |
Aprataxin is a DNA-binding protein involved in different types of DNA break repair. The enzyme acts (among other activities) on abortive DNA ligation intermediates that contain an adenylate covalently linked to the 5′-phosphate DNA terminus. It also acts when the adenylate is covalently linked to the 5′-phosphate of a ribonucleotide linked to a DNA strand, which is the result of abortive ligase activty on products of EC?3.1.26.4, ribonuclease H, an enzyme that cleaves RNA-DNA hybrids on the 5′ side of the ribonucleotide found in the 5′-RNA-DNA-3′ junction. Aprataxin binds the adenylate group to a histidine residue within the active site, followed by its hydrolysis from the nucleic acid and eventual release, leaving a 5′-phosphate terminus that can be efficiently rejoined. The enzyme also possesses the activities of EC?3.1.11.8, 5′-(5′-diphosphoguanosine)-DNA diphosphatase, and EC?3.1.12.2, DNA-3′-(5′-diphosphoguanosine) diphosphatase. |
| Cofactor |
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| History |
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| Reactions |
(1) Adenosine-5′-diphospho-5′-(DNA) + H(2)O = Amp + phospho-5′-(DNA). (2) Adenosine-5′-diphospho-5′-(ribonucleotide)-(DNA) + H(2)O = Amp + 5′-phospho-(ribonucleotide)-(DNA). |
