| 英文名 |
aromatic aldoxime N-monooxygenase |
| Example Structure |
 |
| 别名 |
At4g31500, CYP83B1, cytochrome P450 83B1, HM347235 |
| EC Number |
1.14.14.45 |
| CAS编号 |
|
| Comments |
This cytochrome P450 (heme-thiolate) enzyme is involved in the biosynthesis of glucosinolates in plants. The enzyme catalyses the N-hydroxylation of aromatic aldoximes derived from L-tryptophan and L-phenylalanine, forming an aci-nitro intermediate that reacts non-enzymically with glutathione to produce an N-alkyl-thiohydroximate adduct, the committed precursor of glucosinolates. In the absence of glutathione, the enzyme is suicidal, probably due to interaction of the reactive aci-nitro compound with catalytic residues in the active site. |
| Cofactor |
heme-thiolate |
| History |
|
| Reactions |
(1) (E)-indol-3-ylacetaldehyde oxime + [reduced NADPH–hemoprotein reductase] + glutathione + O(2) = S-((E)-N-hydroxy(indol-3- yl)acetimidoyl)-L-glutathione + [oxidized NADPH–hemoprotein reductase] + 2 H(2)O. (2) (E)-phenylacetaldehyde oxime + [reduced NADPH–hemoprotein reductase] + glutathione + O(2) = S-((Z)-N-hydroxy(phenyl)acetimidoyl)-L-glutathione + [oxidized NADPH–hemoprotein reductase] + 2 H(2)O (overall reaction). |
