Biotinylated Human ANGPT2/Angiopoietin-2 Protein, Accession#: O15123, CAS#: 3202-706-1460; 凯望编码 (ChemWhat Code): 1413965

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Lys275-Phe496.
Biotinylated Yes

Specification

Target ANGPT2/Angiopoietin-2
别名 AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
Gene/Protein ID (Accession) O15123
Species Human
Exact Sequence Lys275-Phe496
分子量 The protein has a predicted MW of 28.4 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-ANGPT2 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPT2, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1413965> 95% as determined by Tris-Bis PAGE
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