Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling), Accession#: P32970, CAS#: 3202-706-431; 凯望编码 (ChemWhat Code): 1412477
Overview
| Product Description | Recombinant Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-terminal.It contains Leu50-Pro193. |
| Biotinylated | Yes |
Specification
| Target | CD27 Ligand/CD70 Trimer |
| 别名 | CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG; Ki-24 antigen; TNFSF7G |
| Gene/Protein ID (Accession) | P32970 |
| Species | Human |
| Exact Sequence | Leu50-Pro193 |
| 分子量 | The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer) , His Tag with the EC50 of 9.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1412477 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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其他供应商 | |
| Watson International Limited | 访问Watson官网 |
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