Biotinylated Human GDF15 Protein, Accession#: Q99988, CAS#: 3202-706-527; 凯望编码 (ChemWhat Code): 1412648

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Human GDF15 Protein is expressed from E.coli with His tag and Avi tag at the N-terminal.It contains Ala197-Ile308.
Biotinylated Yes

Specification

Target Biotinylated GDF15
别名 GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta
Gene/Protein ID (Accession) Q99988
Species Human
Exact Sequence Ala197-Ile308
分子量 The protein has a predicted MW of 15.19 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human GFRAL, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 17.5ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System E.coli
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1412648> 95% as determined by Tris-Bis PAGE
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