Biotinylated Human IL-3 Protein, Accession#: P08700, CAS#: 3202-706-529; 凯望编码 (ChemWhat Code): 1412652
Overview
| Product Description | Recombinant Biotinylated Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-terminal.It contains Ala20-Phe152. |
| Biotinylated | Yes |
Specification
| Target | IL-3 |
| 别名 | IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF |
| Gene/Protein ID (Accession) | P08700 |
| Species | Human |
| Exact Sequence | Ala20-Phe152 |
| 分子量 | The protein has a predicted MW of 16.9 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Human IL-3, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 74.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1412652 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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其他供应商 | |
| Watson International Limited | 访问Watson官网 |
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