Biotinylated Human KRAS G12V (HLA-A*03:01) Protein, Accession#: NP_002107, CAS#: 3202-706-1380; 凯望编码 (ChemWhat Code): 1413875
Overview
| Product Description | Recombinant Biotinylated Human KRAS G12V (HLA-A*03:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide. |
| Biotinylated | Yes |
Specification
| Target | KRAS G12V (HLA-A*03:01) |
| 别名 | |
| Gene/Protein ID (Accession) | NP_002107 |
| Species | Human |
| Exact Sequence | Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M)and VVVGAVGVGK peptide |
| 分子量 | The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1413875 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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其他供应商 | |
| Watson International Limited | 访问Watson官网 |
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