Biotinylated Human M-CSF R/CSF1R/CD115 Protein, Accession#: P07333, CAS#: 3202-706-360; 凯望编码 (ChemWhat Code): 1412339

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Human M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Ile20-Glu512.
Biotinylated Yes

Specification

Target M-CSF R/CSF1R/CD115
别名 CD115; CSF1R; CSF-1-R; CSFR; FMSFIM2; M-CSF R; MCSFR; M-CSFR; C-FMS; FIM2; FMS; HDLS
Gene/Protein ID (Accession) P07333
Species Human
Exact Sequence Ile20-Glu512
分子量 The protein has a predicted MW of 57.4 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-CSF R Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human M-CSF R, His Tag with the EC50 of 27.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.81 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412339> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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