Biotinylated Human SOST/Sclerostin Protein, Accession#: Q9BQB4, CAS#: 3202-706-1109; 凯望编码 (ChemWhat Code): 1413545

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-terminal.It contains Gln24-Tyr213.
Biotinylated Yes

Specification

Target SOST/Sclerostin
别名 sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH
Gene/Protein ID (Accession) Q9BQB4
Species Human
Exact Sequence Gln24-Tyr213
分子量 The protein has a predicted MW of 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-SOST Antibody at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1413545> 95% as determined by Tris-Bis PAGE
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