Biotinylated Human TRAIL R4/TNFRSF10D Protein, Accession#: Q9UBN6, CAS#: 3202-706-1003; 凯望编码 (ChemWhat Code): 1413393

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Human TRAIL R4/TNFRSF10D Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Ala56-His211.
Biotinylated Yes

Specification

Target TRAIL R4/TNFRSF10D
别名 CD264; RSF10D; TRAILR4; DCR2; TRUNDD; TNFRSF10D
Gene/Protein ID (Accession) Q9UBN6
Species Human
Exact Sequence Ala56-His211
分子量 The protein has a predicted MW of 19.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 0.135 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413393> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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