Biotinylated Mouse GDF15 Protein (Primary Amine Labeling), Accession#: Q9Z0J7, CAS#: 3202-706-1667; 凯望编码 (ChemWhat Code): 1414183

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Mouse GDF15 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminal.It contains Ser189-Ala303.
Biotinylated Yes

Specification

Target GDF15
别名 GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta
Gene/Protein ID (Accession) Q9Z0J7
Species Mouse
Exact Sequence Ser189-Ala303
分子量 The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse GFRAL, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GDF15, hFc Tag with the EC50 of 10ng/ml determined by ELISA. See testing image for detail.
Product Tag N-hFc
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1414183> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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