Biotinylated Rat MASP2 Protein (Primary Amine Labeling), Accession#: A2VCV7, CAS#: 3202-706-454; 凯望编码 (ChemWhat Code): 1412513

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Biotinylated Rat MASP2 Protein (Primary Amine Labeling) is expressed from E.coli with His tag at the C-terminal.It contains Thr287-Phe685.
Biotinylated Yes

Specification

Target MASP2
别名 MAP19; MASP-2; MASP1P1; sMap
Gene/Protein ID (Accession) A2VCV7
Species Rat
Exact Sequence Thr287-Phe685
分子量 The protein has a predicted MW of 46.7 kDa. Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-MASP2 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Rat MASP2, His Tag with the EC50 of 19.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System E.coli
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22um filtered solution in 50mM Tris, 200mM NaCl (pH 9.0).
Reconstitution
Stability and Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412513> 95% as determined by Tris-Bis PAGE
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