| 英文名 |
chondroitin-sulfate-ABC endolyase |
| Example Structure |
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| 别名 |
Ch’ase, Ch’ase ABC, chABC, chondroitinase ABC, chondroitinase ABC I, chondroitinase AC, ChS ABC lyase I, EC 4.2.2.4 |
| EC Number |
4.2.2.20 |
| CAS编号 |
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| Comments |
This enzyme degrades a variety of glycosaminoglycans of the chondroitin-sulfate- and dermatan-sulfate type. Chondroitin sulfate, chondroitin-sulfate proteoglycan and dermatan sulfate are the best substrates but the enzyme can also act on hyaluronan at a much lower rate. Keratan sulfate, heparan sulfate and heparin are not substrates. In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. The repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and?N-acetylgalactosamine (GalNAc) [-4)GlcA(¦Â1-3)GalNAc(¦Â1-]n, which may be?O-sulfated on the C-4 and/or C-6 of GalNAc and C-2 of GlcA. GlcA residues of CS may be epimerized to iduronic acid (IdoA) forming the repeating disaccharide [-4)IdoA(¦Á1-3)GalNAc(¦Â1-]n?of DS. Both the concentrations and locations of sulfate-ester substituents vary with glucosaminoglycan source [5]. The related enzyme EC?4.2.2.21, chondroitin-sulfate-ABC exolyase, has the same substrate specificity but removes disaccharide residues from the non-reducing ends of both polymeric chondroitin sulfates and their oligosaccharide fragments produced by EC?4.2.2.20?[4]. |
| Cofactor |
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| History |
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| Reactions |
Endolytic cleavage of (1->4)-beta-galactosaminic bonds between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid to produce a mixture of Delta(4)-unsaturated oligosaccharides of different sizes that are ultimately degraded to Delta(4)-unsaturated tetra- and disaccharides. |
