Cynomolgus APRIL/TNFSF13 Trimer Protein, Accession#: A0A2K5TJA1, CAS#: 3202-706-256; 凯望编码 (ChemWhat Code): 1412225

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-terminal.It contains Lys112-Leu250.
Biotinylated

Specification

Target APRIL/TNFSF13 Trimer
别名 CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
Gene/Protein ID (Accession) A0A2K5TJA1
Species Cynomolgus
Exact Sequence Lys112-Leu250
分子量 The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi,N-Flag
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412225> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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