Cynomolgus CD73/NT5E Protein, Accession#: EHH53214, CAS#: 3202-706-2415; 凯望编码 (ChemWhat Code): 1414905
Overview
| Product Description | Recombinant Cynomolgus CD73/NT5E Protein is expressed from HEK293 with His tag at the C-terminal.It contains Trp27-Cys554. |
| Biotinylated |
Specification
| Target | CD73/NT5E |
| 别名 | CD73; NT5E; NTE; 5′ nucleotidase (CD73); 5′-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55′-nucleotidase; NT5; NTE; CALJA |
| Gene/Protein ID (Accession) | EHH53214 |
| Species | Cynomolgus |
| Exact Sequence | Trp27-Cys554 |
| 分子量 | The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus CD73, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1414905 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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其他供应商 | |
| Watson International Limited | 访问Watson官网 |
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