Cynomolgus CTLA-4/CD152 Protein, Accession#: G7PL88, CAS#: 3202-706-1937; 凯望编码 (ChemWhat Code): 1414431

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Cynomolgus CTLA-4/CD152 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ala37-Ser160.
Biotinylated

Specification

Target CTLA-4/CD152
别名 CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
Gene/Protein ID (Accession) G7PL88
Species Cynomolgus
Exact Sequence Ala37-Ser160
分子量 The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CTLA-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test). The affinity constant of 9.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1414431> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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