Cynomolgus GFRAL/GFR alpha-like Protein, Accession#: XP_015304775, CAS#: 3202-706-958; 凯望编码 (ChemWhat Code): 1413321

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Gln20-Glu351.
Biotinylated

Specification

Target GFRAL/GFR alpha-like
别名 GFR alpha-like; GFRAL; GRAL; C6orf144
Gene/Protein ID (Accession) XP_015304775
Species Cynomolgus
Exact Sequence Gln20-Glu351
分子量 The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM PB, 0.4M NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM PB, 0.4M NaCl (pH 6.0).
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413321> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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