Cynomolgus PLAU/uPA Protein (active form), Accession#: A0A2K5WND1, CAS#: 3202-706-254; 凯望编码 (ChemWhat Code): 1412221
Overview
| Product Description | Recombinant Cynomolgus PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-terminal.It contains Ser21-Leu430, which consists of two chains: Long chain A and chain B. The long chain A is further cleaved to yield a short chain A and N-terminal fragment. |
| Biotinylated |
Specification
| Target | PLAU/uPA |
| 别名 | |
| Gene/Protein ID (Accession) | A0A2K5WND1 |
| Species | Cynomolgus |
| Exact Sequence | Ser21-Leu430 |
| 分子量 | The protein has a predicted MW of 47.23 kDa. Due to protein lysis and glycosylation, the protein migrates to 23-25 kDa (long chain A), 35-40 kDa (chain B) and 50-60 kDa (long chain A&chain B) based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus PLAU, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 25.1ng/ml determined by ELISA. The affinity constant of 0.033 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1412221 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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其他供应商 | |
| Watson International Limited | 访问Watson官网 |
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