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Aprataxin is a DNA-binding protein that catalyses (among other activities) the 3′ decapping of DNA-ppG (formed by EC?6.5.1.8, 3′-phosphate/5′-hydroxy nucleic acid ligase) [1]. The enzyme binds the guanylate group to a histidine residue at its active site, forming a covalent enzyme-nucleotide phosphate intermediate, followed by the hydrolysis of the guanylate from the nucleic acid and its eventual release. The enzyme also possesses the activity of EC?3.1.11.7, DNA-(5′-diphosphoadenosine) diphosphatase, and EC?3.1.11.8, 5′-diphosphoguanosinylyl-(3′-5′)-[DNA] diphosphatase. |
