Human B7-H4 Protein, Accession#: Q7Z7D3, CAS#: 3202-706-1261; 凯望编码 (ChemWhat Code): 1413733

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Phe29-Ala258.
Biotinylated

Specification

Target B7-H4
别名 B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291
Gene/Protein ID (Accession) Q7Z7D3
Species Human
Exact Sequence Phe29-Ala258
分子量 The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human B7-H4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413733> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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