Human BDCA-2 Protein, Accession#: Q8WTT0, CAS#: 3202-706-406; 凯望编码 (ChemWhat Code): 1412429

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human BDCA-2 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Phe46-Ile213.
Biotinylated

Specification

Target BDCA-2
别名 BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303; BDCA2MGC125791; CD303; CLECSF11PRO34150; DLEC; DLECMGC125792; HECLMGC125793; MGC125789; PRO34150
Gene/Protein ID (Accession) Q8WTT0
Species Human
Exact Sequence Phe46-Ile213
分子量 The protein has a predicted MW of 22.7 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human BDCA-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Endotoxin Less than 1EU per μg by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 200mM L-Arginine (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412429> 95% as determined by Tris-Bis PAGE
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