Human CD30 Ligand/TNFSF8 Protein, Accession#: P32971, CAS#: 3202-706-815; 凯望编码 (ChemWhat Code): 1413083

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-terminal.It contains Gln63-Asp234.
Biotinylated

Specification

Target CD30 Ligand/TNFSF8
别名 CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG
Gene/Protein ID (Accession) P32971
Species Human
Exact Sequence Gln63-Asp234
分子量 The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD30, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc (IgG2a) Tag with the EC50 of 31.9ng/ml determined by ELISA. See testing image for detail.
Product Tag N-mFc
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1413083> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC;
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