Human CD40 Ligand/TNFSF5 Trimer Protein, Accession#: P29965, CAS#: 3202-706-1101; 凯望编码 (ChemWhat Code): 1413537
Overview
| Product Description | Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-terminal.It contains Met113-Leu261. |
| Biotinylated |
Specification
| Target | CD40 Ligand/TNFSF5 Trimer |
| 别名 | CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
| Gene/Protein ID (Accession) | P29965 |
| Species | Human |
| Exact Sequence | Met113-Leu261 |
| 分子量 | The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD40 Ligand Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 1.29ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Flag |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1413537 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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其他供应商 | |
| Watson International Limited | 访问Watson官网 |
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