Human/Cynomolgus/Rhesus macaque CD28 Protein, Accession#: P10747, CAS#: 3202-706-1015; 凯望编码 (ChemWhat Code): 1413415

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-terminal.It contains Asn19-Pro152.
Biotinylated

Specification

Target CD28
别名 CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44
Gene/Protein ID (Accession) P10747
Species Human/Cynomolgus/Rhesus macaque
Exact Sequence Asn19-Pro152
分子量 The protein has a predicted MW of 41.9 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA. See testing image for detail.
Product Tag N-mFc
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1413415> 95% as determined by Tris-Bis PAGE
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