Human E-Selectin/CD62E Protein, Accession#: P16581, CAS#: 3202-706-1557; 凯望编码 (ChemWhat Code): 1414071
Overview
| Product Description | Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Trp22-Pro556. |
| Biotinylated |
Specification
| Target | E-Selectin/CD62E |
| 别名 | CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2 |
| Gene/Protein ID (Accession) | P16581 |
| Species | Human |
| Exact Sequence | Trp22-Pro556 |
| 分子量 | The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human E-Selectin, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1414071 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| 没有试剂厂商? | 向凯望发送快速询价 |
| 想要被列为试剂厂商? (付费服务) | 点击这里联系凯望 |
认证生产商 | |
| 想要被列为认证生产商 (需要通过认证)? | 请下载并填写 这份表格并发邮件到approved-manufacturers@chemwhat.com |
其他供应商 | |
| Watson International Limited | 访问Watson官网 |
如需其他帮助，请联系我们 | |
| 联系我们获取其它信息或服务 | 点击这里联系凯望 |