Human EFEMP1 Protein, Accession#: Q12805, CAS#: 3202-706-363; 凯望编码 (ChemWhat Code): 1412345

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human EFEMP1 Protein is expressed from HEK293 with His tag at the N-terminal.It contains Gln18-Phe493.
Biotinylated

Specification

Target EFEMP1
别名 EFEMP1; FBLN3; FBNL; DHRD; FBNLFLJ35535; FIBL-3; fibrillin-like; Fibulin 3; MGC111353; MLVT; MTLV; S1-5
Gene/Protein ID (Accession) Q12805
Species Human
Exact Sequence Gln18-Phe493
分子量 The protein has a predicted MW of 53.9 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human EFEMP1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 35.1ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412345> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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