Human EGF Protein, Accession#: P01133, CAS#: 3202-706-366; 凯望编码 (ChemWhat Code): 1412349
Overview
| Product Description | Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023. |
| Biotinylated |
Specification
| Target | EGF |
| 别名 | HOMG4; EGF; URG |
| Gene/Protein ID (Accession) | P01133 |
| Species | Human |
| Exact Sequence | Asn971-Arg1023 |
| 分子量 | The protein has a predicted MW of 5.6 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Human EGF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.3ug/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | No Tag |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1412349 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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其他供应商 | |
| Watson International Limited | 访问Watson官网 |
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