Human FGF21 Protein, Accession#: Q9NSA1, CAS#: 3202-706-1601; 凯望编码 (ChemWhat Code): 1414113
Overview
| Product Description | Recombinant Human FGF21 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at the N-terminal.It contains His29-Ser209. |
| Biotinylated |
Specification
| Target | FGF21 |
| 别名 | UNQ3115; PRO10196; FGF-21; FGF21 |
| Gene/Protein ID (Accession) | Q9NSA1 |
| Species | Human |
| Exact Sequence | His29-Ser209 |
| 分子量 | The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human FGF21 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 2.29ug/ml determined by ELISA (QC Test). The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-mFc-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1414113 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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其他供应商 | |
| Watson International Limited | 访问Watson官网 |
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