Human Galectin 3 Protein, Accession#: P17931, CAS#: 3202-706-1359; 凯望编码 (ChemWhat Code): 1413853

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human Galectin 3 Protein is expressed from HEK293 with His tag at the N-terminal.It contains Ala2-Ile250.
Biotinylated

Specification

Target Galectin 3
别名 AGE-R3; CBP35; GAL3; Gal-3; galactin-3; GALBPCBP35; Galectin3; GALIG; L29; L31; Lectin L-29; LGALS2; LGALS3; Mac-2; MAC2GAL3
Gene/Protein ID (Accession) P17931
Species Human
Exact Sequence Ala2-Ile250
分子量 The protein has a predicted MW of 27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Galectin 3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA (QC Test). The affinity constant of 33.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM HEPES, 150mM NaCl, 5mM EDTA, 2mM TCEP (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413853> 95% as determined by Tris-Bis PAGE
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