Human HGF Protein, Accession#: P14210, CAS#: 3202-706-386; 凯望编码 (ChemWhat Code): 1412389

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human HGF Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gln32-Ser728.
Biotinylated

Specification

Target HGF
别名 Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB
Gene/Protein ID (Accession) P14210
Species Human
Exact Sequence Gln32-Ser728
分子量 The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Activity Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Endotoxin Less than 1EU per μg by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 300mM Arginine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1412389> 95% as determined by Tris-Bis PAGE
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