Human IL-10 Protein, Accession#: P22301, CAS#: 3202-706-951; 凯望编码 (ChemWhat Code): 1413311

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human IL-10 Protein is expressed from HEK293 without tag.It contains Ser19-Asn178.
Biotinylated

Specification

Target IL-10
别名 Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451
Gene/Protein ID (Accession) P22301
Species Human
Exact Sequence Ser19-Asn178
分子量 The protein has a predicted MW of 18.8 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 21.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.255 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag No Tag
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1413311> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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