Human IL-7 Protein, Accession#: P13232, CAS#: 3202-706-802; 凯望编码 (ChemWhat Code): 1413061

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
Biotinylated

Specification

Target IL-7
别名 IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
Gene/Protein ID (Accession) P13232
Species Human
Exact Sequence Asp26-His177
分子量 The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml determined by ELISA. The affinity constant of 22.66 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag No Tag
Express System E.coli
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 25mM PB, 250mM NaCl (pH 6.8).
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413061> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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