Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein, Accession#: P11215, CAS#: 3202-706-810; 凯望编码 (ChemWhat Code): 1413075

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-terminal.It contains Phe17-Asn1104(ITGAM) acidic tail&Gln23-Asn700(ITGB2) basic tail.
Biotinylated

Specification

Target Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer
别名
Gene/Protein ID (Accession) P11215
Species Human
Exact Sequence Phe17-Asn1104(ITGAM)acidic tail & Gln23-Asn700(ITGB2)basic tail
分子量 The protein has a predicted MW of 126.10 kDa(ITGAM)&79.50 kDa(ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM)&90-100 kDa (ITGB2) based on Tris-Bis PAGE result.
Activity Immobilized Human ITGAM&ITGB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413075> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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