Human LRP-5 Protein, Accession#: O75197, CAS#: 3202-706-753; 凯望编码 (ChemWhat Code): 1412977

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human LRP-5 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminal.It contains Glu644-Gln1263.
Biotinylated

Specification

Target LRP-5
别名 LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2
Gene/Protein ID (Accession) O75197
Species Human
Exact Sequence Glu644-Gln1263
分子量 The protein has a predicted MW of 96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human DKK1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
Product Tag C-mFc
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412977> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
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