Human LRP-6 Protein, Accession#: O75581, CAS#: 3202-706-936; 凯望编码 (ChemWhat Code): 1413287

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human LRP-6 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminal.It contains Ala20-Pro630.
Biotinylated

Specification

Target LRP-6
别名 LRP-6; DCAD2; FLJ90062; FLJ90421
Gene/Protein ID (Accession) O75581
Species Human
Exact Sequence Ala20-Pro630
分子量 The protein has a predicted MW of 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/Well) on plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 0.87ug/ml determined by ELISA. See testing image for detail.
Product Tag C-mFc
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1413287> 90% as determined by Tris-Bis PAGE
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