Human NGAL/Lipocalin-2 Protein, Accession#: P80188, CAS#: 3202-706-456; 凯望编码 (ChemWhat Code): 1412517

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Gln21-Gly198.
Biotinylated

Specification

Target NGAL/Lipocalin-2
别名 NGAL; Lipocalin-2; Oncogene 24p3; p25; Siderocalin LCN2; MSFI
Gene/Protein ID (Accession) P80188
Species Human
Exact Sequence Gln21-Gly198
分子量 The protein has a predicted MW of 47.2 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human NGAL, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody, hFc Tag with the EC50 19.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM MES,150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES,150mM NaCl (pH 6.5).
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412517> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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