Human Notch 3 Protein, Accession#: Q9UM47, CAS#: 3202-706-767; 凯望编码 (ChemWhat Code): 1413001
Overview
| Product Description | Recombinant Human Notch 3 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ala1378-Ser1640. |
| Biotinylated |
Specification
| Target | Notch 3 |
| 别名 | |
| Gene/Protein ID (Accession) | Q9UM47 |
| Species | Human |
| Exact Sequence | Ala1378-Ser1640 |
| 分子量 | The protein has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1413001 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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其他供应商 | |
| Watson International Limited | 访问Watson官网 |
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