Human SEMA3A Protein, Accession#: Q14563, CAS#: 3202-706-1304; 凯望编码 (ChemWhat Code): 1413781

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human SEMA3A Protein is expressed from HEK293 with His tag at the N-terminal.It contains Asn21-Val771.
Biotinylated

Specification

Target SEMA3A
别名 Semaphorin-3A; Semaphorin III; Sema III; Semaphorin-D; Sema D; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; SEMA1, Sema3A; SEMAD; SEMAIII; SEMAL; semaphorin L
Gene/Protein ID (Accession) Q14563
Species Human
Exact Sequence Asn21-Val771
分子量 The protein has a predicted MW of 87.7 kDa same as Tris-Bis PAGE result.
Activity Immobilized Anti-SEMA3A Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 0.62ug/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413781> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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