Human Siglec-15/CD33L3 Protein, Accession#: Q6ZMC9, CAS#: 3202-706-757; 凯望编码 (ChemWhat Code): 1412985

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Phe20-Thr263.
Biotinylated

Specification

Target Siglec-15/CD33L3
别名 CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
Gene/Protein ID (Accession) Q6ZMC9
Species Human
Exact Sequence Phe20-Thr263
分子量 The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0).
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1412985> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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