Human SIRP alpha V3 Protein, Accession#: ATD50864, CAS#: 3202-706-1025; 凯望编码 (ChemWhat Code): 1413429

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human SIRP alpha V3 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Glu31-Arg369(H54L).
Biotinylated

Specification

Target SIRP alpha V3
别名 CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
Gene/Protein ID (Accession) ATD50864
Species Human
Exact Sequence Glu31-Arg369(H54L)
分子量 The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human SIRP alpha V3, His tag at 1ug/ml (100ul/Well). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.7ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413429> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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