Human VEGF-C/Flt4-L Protein, Accession#: Q6FH59, CAS#: 3202-706-974; 凯望编码 (ChemWhat Code): 1413351

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Thr103-Arg227.
Biotinylated

Specification

Target VEGF-C/Flt4-L
别名 VEGFC; VEGFc; Flt4-L; VRP; VEGF-C
Gene/Protein ID (Accession) Q6FH59
Species Human
Exact Sequence Thr103-Arg227
分子量 The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCI (pH 6.0).
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

试剂采购

ChemWhat 1413351> 95% as determined by Tris-Bis PAGE
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