Human VEGF R3/FLT4 Protein, Accession#: P35916, CAS#: 3202-706-759; 凯望编码 (ChemWhat Code): 1412989

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human VEGF R3/FLT4 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Tyr25-Ile776.
Biotinylated

Specification

Target VEGF R3/FLT4
别名 VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL
Gene/Protein ID (Accession) P35916
Species Human
Exact Sequence Tyr25-Ile776
分子量 The protein has a predicted MW of 111.3 kDa. Due to glycosylation, the protein migrates to 135-155 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl, 100mM Glycine (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412989> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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