| Comments |
This enzyme, along with?N-carbamoylase (EC?3.5.1.77?and EC?3.5.1.87) and hydantoinase, forms part of the reaction cascade known as the “hydantoinase process”, which allows the total conversion of?D,L-5-monosubstituted hydantoins into optically pure?D- or?L-amino acids [7]. The enzyme from?Pseudomonas?sp. (HyuE) has a preference for hydantoins with aliphatic substituents, e.g.?D- and?L-5-[2-(methylsulfanyl)ethyl]hydantoin, whereas that from?Arthrobacter aurescens?shows highest activity with arylalkyl substituents, especially 5-benzylhydantoin, at the 5-position [2]. In the enzyme from?Sinorhizobium meliloti, Cys76?is responsible for recognition and proton retrieval of?D-isomers, while Cys181?is responsible for?L-isomer recognition and racemization [6]. |
