| Comments |
The enzymes from the bacteria?Pseudomonas aeruginosa?and?Xenorhabdus nematophila?are involved in the biosynthesis of paerucumarin and rhabduscin, respectively. According to the proposed mechanism, the enzyme forms an imine intermediate composed of linked?L-tyrosine and?D-ribulose 5-phosphate, followed by loss of the phosphate group and formation of a ¦Â-keto imine and keto-enol tautomerization. This is followed by a C-C bond cleavage, the release of acetol, and a retro aldol type reaction that releases formaldehyde and forms the final product [3].?cf. EC?4.1.99.25,?L-tryptophan isonitrile synthase. |
