| 英文名 |
(methylsulfanyl)alkanaldoxime N-monooxygenase |
| Example Structure |
 |
| 别名 |
(methylthio)alkanaldoxime N-monooxygenase, CYP83A1, CYP83A1.1, CYP83B1, cytochrome P450 CYP83A1, cytochrome P450 monooxygenase 83A1-2 |
| EC Number |
1.14.14.43 |
| CAS编号 |
|
| Comments |
This cytochrome P-450 (heme thiolate) enzyme is involved in the biosynthesis of glucosinolates in plants. The enzyme catalyses an N-hydroxylation of the E isomer of n-(methylsulfanyl)aldoximes, forming an aci-nitro intermediate that reacts non-enzymically with glutathione to produce an N-alkyl-thiohydroximate adduct, the committed precursor of glucosinolates. In the absence of a thiol compound, the enzyme is suicidal, probably due to interaction of the reactive aci-nitro intermediate with active site residues. |
| Cofactor |
heme-thiolate |
| History |
|
| Reactions |
An (E)-omega-(methylthio)alkanal oxime + O(2) + glutathione + [reduced NADPH–hemoprotein reductase] = an (E)-1-(glutathione-S-yl)-omega- (methylthio)-alkylhydroximate + 2 H(2)O + [oxidized NADPH–hemoprotein reductase]. |
