Mouse RNF43 Protein, Accession#: Q5NCP0, CAS#: 3202-706-1368; 凯望编码 (ChemWhat Code): 1413863
Overview
| Product Description | Recombinant Mouse RNF43 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Gly24-Tyr197. |
| Biotinylated |
Specification
| Target | RNF34 |
| 别名 | E3 ubiquitin-protein ligase RNF43；RING finger protein 43；RING-type E3 ubiquitin transferase RNF43；Rnf43 |
| Gene/Protein ID (Accession) | Q5NCP0 |
| Species | Mouse |
| Exact Sequence | Gly24-Tyr197 |
| 分子量 | The protein has a predicted MW of 45.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Mouse R spondin 1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.40ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
试剂采购 | |
| ChemWhat 1413863 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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| Watson International Limited | 访问Watson官网 |
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