Rat GDF15 Protein, Accession#: Q9Z0J6, CAS#: 3202-706-1277; 凯望编码 (ChemWhat Code): 1413751

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Rat GDF15 Protein is expressed from E.coli with His tag at the N-terminal.It contains Ser189-Ala303.
Biotinylated

Specification

Target GDF15
别名
Gene/Protein ID (Accession) Q9Z0J6
Species Rat
Exact Sequence Ser189-Ala303
分子量 The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Tris-Bis PAGE result.
Activity Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 6.2ug/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System E.coli
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413751> 95% as determined by Tris-Bis PAGE
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