| Official Full Name |
Recombinant Bovine Enterokinase Light Chain, His (rBoEK,His) |
| Squence |
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| Amino Acid Sequence |
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| 别名 |
Enterokinase, Serine Protease 7, Transmembrane Protease Serine 15 |
| Accession Number |
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| GeneID |
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| Summary |
Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfide-linked 82–140 kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 kDa light chain which contains the catalytic subunit. Additionally, both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue.
rEK was report to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK.
rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications. rBoEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system. |
| Source |
Escherichia coli. |
| 分子量 |
Approximately 28 kDa, a single non-glycosylated polypeptide chain containing 241 amino acids，with 6 × His at C-terminus. |
| Biological Activity |
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| Appearance |
Sterile liquid. |
| Formulation |
50 mM Tris-HCl, pH 8.0, 0.5 M NaCl and 50 % glycerol. |
| Endotoxin |
Less than 1 EU/ug of rBoEKL, His as determined by LAL method. |
| Reconstitution |
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| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening. |
| References |
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| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
