| Official Full Name |
Recombinant Human Beta-defensin 3 (rHuBD-3) |
| Squence |
 |
| Amino Acid Sequence |
GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK |
| 别名 |
Defensin beta3 |
| Accession Number |
P81534 |
| GeneID |
55894 |
| Summary |
|
| Source |
Escherichia coli. |
| 分子量 |
Approximately 5.2 kDa, a single non-glycosylated polypeptide chain containing 45 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 ug/ml, corresponding to a specific activity of > 33.3 IU/mg. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
| Endotoxin |
Less than 1 EU/ug of rHuBD-3 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Lioi AB, Reyes Rodriguez AL, Funderburg NT, et al. 2012. J Leukoc Biol, 92: 1083-91.2. Wang K, Wang JH, Baskaran H, et al. 2012. Head Neck Oncol, 4: 41.3. Lee JK, Park YJ, Kum KY, et al. 2012. Int Endod J, |
| SDS-PAGE |
 |
| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
